pCI neo-hEST2

LOCUS       pCI neo-hEST2                8898 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCI neo-hEST2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddle SD, 
            Ziaugra L, Beijersbergen RL, Davidoff MJ, Liu Q, Bacchetti S, Haber 
            DA, Weinberg RA
  TITLE     hEST2, the putative human telomerase catalytic subunit gene, is 
            up-regulated in tumor cells and during immortalization.
  JOURNAL   Cell 1997 Aug 22;90(4):785-95.
  PUBMED    9288757
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      108..696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     597..616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        907..1286
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1287..1498
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1449..1469
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1495..1519
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1659..1791
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1774..1793
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1836..1854
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1916..5314
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     promoter        complement(5336..5353)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    5371..5492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5382..5401)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5436..5455
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5678..6133
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5765..5784)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5975..5996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6250..6607
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(6273..6293)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6458..6593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6520..6539
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6658..7452
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6712..6731)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7322..7341
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7516..7564
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7605..7624)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7724..7746
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7784..7802)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7870..7974
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7975..8835
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8193..8212)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
       61 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
      121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
      181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
      241 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
      301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      721 cggttcctgg ccttttgctg gccttttgct cacatggctc gacagatctt caatattggc
      781 cattagccat attattcatt ggttatatag cataaatcaa tattggctat tggccattgc
      841 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
      901 catgttggca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      961 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     1021 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     1081 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     1141 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
     1201 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
     1261 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
     1321 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     1381 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactgc gatcgcccgc
     1441 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     1501 tttagtgaac cgtcagatca ctagaagctt tattgcggta gtttatcaca gttaaattgc
     1561 taacgcagtc agtgcttctg acacaacagt ctcgaactta agctgcagtg actctcttaa
     1621 ggtagccttg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac
     1681 aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct
     1741 gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact
     1801 cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc
     1861 tcgagaattc gtcctgctgc gcacgtggga agccctggcc ccggccaccc ccgcgatgcc
     1921 gcgcgctccc cgctgccgag ccgtgcgctc cctgctgcgc agccactacc gcgaggtgct
     1981 gccgctggcc acgttcgtgc ggcgcctggg gccccagggc tggcggctgg tgcagcgcgg
     2041 ggacccggcg gctttccgcg cgctggtggc ccagtgcctg gtgtgcgtgc cctgggacgc
     2101 acggccgccc cccgccgccc cctccttccg ccaggtgtcc tgcctgaagg agctggtggc
     2161 ccgagtgctg cagaggctgt gcgagcgcgg cgcgaagaac gtgctggcct tcggcttcgc
     2221 gctgctggac ggggcccgcg ggggcccccc cgaggccttc accaccagcg tgcgcagcta
     2281 cctgcccaac acggtgaccg acgcactgcg ggggagcggg gcgtgggggc tgctgctgcg
     2341 ccgcgtgggc gacgacgtgc tggttcacct gctggcacgc tgcgcgctct ttgtgctggt
     2401 ggctcccagc tgcgcctacc aggtgtgcgg gccgccgctg taccagctcg gcgctgccac
     2461 tcaggcccgg cccccgccac acgctagtgg accccgaagg cgtctgggat gcgaacgggc
     2521 ctggaaccat agcgtcaggg aggccggggt ccccctgggc ctgccagccc cgggtgcgag
     2581 gaggcgcggg ggcagtgcca gccgaagtct gccgttgccc aagaggccca ggcgtggcgc
     2641 tgcccctgag ccggagcgga cgcccgttgg gcaggggtcc tgggcccacc cgggcaggac
     2701 gcgtggaccg agtgaccgtg gtttctgtgt ggtgtcacct gccagacccg ccgaagaagc
     2761 cacctctttg gagggtgcgc tctctggcac gcgccactcc cacccatccg tgggccgcca
     2821 gcaccacgcg ggccccccat ccacatcgcg gccaccacgt ccctgggaca cgccttgtcc
     2881 cccggtgtac gccgagacca agcacttcct ctactcctca ggcgacaagg agcagctgcg
     2941 gccctccttc ctactcagct ctctgaggcc cagcctgact ggcgctcgga ggctcgtgga
     3001 gaccatcttt ctgggttcca ggccctggat gccagggact ccccgcaggt tgccccgcct
     3061 gccccagcgc tactggcaaa tgcggcccct gtttctggag ctgcttggga accacgcgca
     3121 gtgcccctac ggggtgctcc tcaagacgca ctgcccgctg cgagctgcgg tcaccccagc
     3181 agccggtgtc tgtgcccggg agaagcccca gggctctgtg gcggcccccg aggaggagga
     3241 cacagacccc cgtcgcctgg tgcagctgct ccgccagcac agcagcccct ggcaggtgta
     3301 cggcttcgtg cgggcctgcc tgcgccggct ggtgccccca ggcctctggg gctccaggca
     3361 caacgaacgc cgcttcctca ggaacaccaa gaagttcatc tccctgggga agcatgccaa
     3421 gctctcgctg caggagctga cgtggaagat gagcgtgcgg ggctgcgctt ggctgcgcag
     3481 gagcccaggg gttggctgtg ttccggccgc agagcaccgt ctgcgtgagg agatcctggc
     3541 caagttcctg cactggctga tgagtgtgta cgtcgtcgag ctgctcaggt ctttctttta
     3601 tgtcacggag accacgtttc aaaagaacag gctctttttc taccggaaga gtgtctggag
     3661 caagttgcaa agcattggaa tcagacagca cttgaagagg gtgcagctgc gggagctgtc
     3721 ggaagcagag gtcaggcagc atcgggaagc caggcccgcc ctgctgacgt ccagactccg
     3781 cttcatcccc aagcctgacg ggctgcggcc gattgtgaac atggactacg tcgtgggagc
     3841 cagaacgttc cgcagagaaa agagggccga gcgtctcacc tcgagggtga aggcactgtt
     3901 cagcgtgctc aactacgagc gggcgcggcg ccccggcctc ctgggcgcct ctgtgctggg
     3961 cctggacgat atccacaggg cctggcgcac cttcgtgctg cgtgtgcggg cccaggaccc
     4021 gccgcctgag ctgtactttg tcaaggtgga tgtgacgggc gcgtacgaca ccatccccca
     4081 ggacaggctc acggaggtca tcgccagcat catcaaaccc cagaacacgt actgcgtgcg
     4141 tcggtatgcc gtggtccaga aggccgccca tgggcacgtc cgcaaggcct tcaagagcca
     4201 cgtctctacc ttgacagacc tccagccgta catgcgacag ttcgtggctc acctgcagga
     4261 gaccagcccg ctgagggatg ccgtcgtcat cgagcagagc tcctccctga atgaggccag
     4321 cagtggcctc ttcgacgtct tcctacgctt catgtgccac cacgccgtgc gcatcagggg
     4381 caagtcctac gtccagtgcc aggggatccc gcagggctcc atcctctcca cgctgctctg
     4441 cagcctgtgc tacggcgaca tggagaacaa gctgtttgcg gggattcggc gggacgggct
     4501 gctcctgcgt ttggtggatg atttcttgtt ggtgacacct cacctcaccc acgcgaaaac
     4561 cttcctcagg accctggtcc gaggtgtccc tgagtatggc tgcgtggtga acttgcggaa
     4621 gacagtggtg aacttccctg tagaagacga ggccctgggt ggcacggctt ttgttcagat
     4681 gccggcccac ggcctattcc cctggtgcgg cctgctgctg gatacccgga ccctggaggt
     4741 gcagagcgac tactccagct atgcccggac ctccatcaga gccagtctca ccttcaaccg
     4801 cggcttcaag gctgggagga acatgcgtcg caaactcttt ggggtcttgc ggctgaagtg
     4861 tcacagcctg tttctggatt tgcaggtgaa cagcctccag acggtgtgca ccaacatcta
     4921 caagatcctc ctgctgcagg cgtacaggtt tcacgcatgt gtgctgcagc tcccatttca
     4981 tcagcaagtt tggaagaacc ccacattttt cctgcgcgtc atctctgaca cggcctccct
     5041 ctgctactcc atcctgaaag ccaagaacgc agggatgtcg ctgggggcca agggcgccgc
     5101 cggccctctg ccctccgagg ccgtgcagtg gctgtgccac caagcattcc tgctcaagct
     5161 gactcgacac cgtgtcacct acgtgccact cctggggtca ctcaggacag cccagacgca
     5221 gctgagtcgg aagctcccgg ggacgacgct gactgccctg gaggccgcag ccaacccggc
     5281 actgccctca gacttcaaga ccatcctgga ctgagtcgac ccgggcggcc gcttcccttt
     5341 agtgagggtt aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     5401 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     5461 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     5521 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     5581 gtaaaatccg ataaggatcg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg
     5641 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     5701 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5761 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5821 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5881 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5941 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6001 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6061 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6121 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     6181 gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
     6241 aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     6301 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6361 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6421 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6481 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6541 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6601 ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaaggctag agccaccatg
     6661 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     6721 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     6781 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     6841 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     6901 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     6961 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     7021 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     7081 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     7141 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     7201 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     7261 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     7321 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     7381 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     7441 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     7501 catcacgatg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     7561 tgtgaatcga tagcgataag gatccgcgta tggtgcactc tcagtacaat ctgctctgat
     7621 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7681 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7741 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     7801 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7861 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7921 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7981 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8041 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8101 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8161 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8221 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8281 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8341 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8401 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8461 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8521 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8581 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8641 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8701 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8761 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8821 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8881 cttcattttt aatttaaa
//